Last data update: 24 January 2024 16:39 CET
Plasmid name: pLenti6(bla)-mPcdh10long-HA (LMBP 12687)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
NGS: e07-gc-may2021.fasta |
| Cloned DNA: |
Mouse protocadherin 10 cDNA (Pcdh10, GeneID 18526) Influenza HA epitope encoding the haemagglutinin tagging peptide; C-terminal |
| Promoter: | Rous sarcoma virus/human immunodeficiency virus hybrid long terminal repeat (RSV/HIV-1 5' LTR) Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Escherichia coli lac operon promoter Simian virus 40 early promoter (SV40 early) Phage T3 promoter Phage T7 gene 10 promoter (T7g10) Synthetic prokaryotic EM7 promoter |
| Ribosome binding site: |
- |
| Terminator: | Rous sarcoma virus/human immunodeficiency virus hybrid long terminal repeat (RSV/HIV-1 3' LTR); modified HIV-1 3' LTR with deleted U3 region (ΔU3/3' LTR) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Blasticidin (bsd) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pLenti6(Blast)HA-Dest |
| Further information: | This lentiviral Gateway expression vector was constructed by cloning the coding sequence of long isoform 4 of mouse Pcdh10 into the pLenti6(Blast)HA-Dest vector via Gateway LR recombination. Because the risk for recombination after each subcultivation is high, this plasmid is only available under the format of isolated plasmid DNA. Other name of the plasmid is pLenti6 (bla)_mpcdh10long Iso 4 (no stop)_HA. |
| EMBL Accession number: | - |
| Latest sequence update: | 24/06/2021 |
| Authenticity test: | This plasmid has been fully sequenced but the NGS sequence data still need to be implemented. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr F. Van Roy (1)(2). It was constructed by E. Sanders (1)(2). (1) UGent-VIB Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Kleinberger et al., BMC Cancer 22 (2022), 451 [PMID: 35468745] [DOI: 10.1186/s12885-022-09381-y] |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αT1R |
| Host reference: | - |
| Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pLenti6(bla)-mPcdh10long-HA (LMBP 12687) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr F. Van Roy . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.