Last data update: 24 January 2024 16:39 CET
Plasmid name: pMC519SAX (LMBP 3485)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Streptomyces avidinii streptavidin gene (SA) - Staphylococcus aureus protein A cDNA (spa) anchor (SA-SPA-anchor, SAX) |
| Promoter: | Escherichia coli hybrid tryptophan/lacUV5 promoter (tac) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli alkaline phosphatase A gene (phoA) |
| Terminator: | - |
| Selection marker: | Chloramphenicol (cam) |
| Replicon: | Escherichia coli plasmid ColE1 origin |
| Host range: | Escherichia coli |
| Further information: | When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. After mutagenesis, use mutS strains for primary transformation (e.g. sup(-) strain WK6mutS, sup(+) strain BMH71-18mutS); for segregation of possible mutants: sup(-) strains (e.g. WK6). |
| EMBL Accession number: | - |
| Latest sequence update: | 06/12/2011 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr L. Steidler(1) (2) and Prof. Dr E. Remaut(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 RR1ZΔM15 |
| Host reference: | Altieri et al., J. Bacteriol. 168 (1986), 648-654 [PMID: 2946661] |
| Cultivation medium: | LB-Lennox + chloramphenicol (25 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pMC519SAX (LMBP 3485) is available at BCCM/GeneCorner. This plasmid was deposited by Dr L. Steidler and Prof. Dr E. Remaut . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.