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GeneCorner plasmid details

Last data update: 24 January 2024 16:39 CET

Plasmid name: pMC7 (LMBP 7980)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: not available
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: -
Ribosome
binding site:
-
Terminator: -
Selection marker: Tetracycline (tet)
ARG4; auxotrophic
Replicon: -
Host range: Escherichia coli
Parental clone: pMB9
Further information: The plasmid was constructed by inserting a 9.5 kb fragment, including 8 kb from the E. coli argECBH+ region, into pMB9.
Total length of the plasmid is approximately 14,9 kb.
This plasmid belongs to the colE1 incompatibility family.
EMBL Accession number: -
Latest sequence update: 18/03/2013
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by M. Mergeay(1) and A. Provoost(1).
(1) SCK.CEN, Mol, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 SAO19-H2
Host reference: -
Cultivation medium: LB-Lennox + tetracycline (10 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: SCK CM0157

Refer in your Materials and Methods:

pMC7 (LMBP 7980) is available at BCCM/GeneCorner. This plasmid was deposited by M. Mergeay and A. Provoost.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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