Last data update: 24 January 2024 16:39 CET
Plasmid name: pMET7-hCRK-FLAG (LMBP 9526)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p9526.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Human v-crk avian sarcoma virus CT10 oncogene homolog cDNA (CRK) FLAG epitope tag; C-terminal |
Promoter: | Simian virus 40 early/human T-cell lymphotropic virus type 1 LTR hybrid promoter (SRα) Phage T7 gene 10 promoter (T7g10) |
Ribosome binding site: |
- |
Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
Parental clone: | pMET7 |
Further information: | The plasmid was constructed by cloning the short isoform of the human CRK coding sequence into the pMET7 vector. The nucleotide sequence of the human CRK coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number BC001718.1. Other name of the plasmid is pMET7.CRK1-Flag. |
EMBL Accession number: | BC001718.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 05/02/2016 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr R. Beyaert(1)(2). It was constructed by Dr C. Müller(1)(2). (1) Inflammation Research Center, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5α |
Host reference: | Focus 8 (1986), 9 |
Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pMET7-hCRK-FLAG (LMBP 9526) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.