Last data update: 24 January 2024 16:39 CET
Plasmid name: pMET7hIL-2Ralpha (LMBP 5280)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: | p5280.gb (View with Genome Compiler) |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human interleukin 2 receptor subunit alpha cDNA (IL2RA, IDDM10, IL2R, CD25, GeneID 3559) |
| Promoter: | Simian virus 40 early/human T-cell lymphotropic virus type 1 LTR hybrid promoter (SRα) Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pMET7 |
| Further information: | The plasmid contains the human interleukin 2 receptor α (hIL2Rα) coding sequence inserted downstream from the Simian virus 40 early/human T-cell lymphotropic virus type 1 LTR hybrid promoter (SRα) and from the phage T7 gene 10 promoter (T7g10). The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727001.1. The nucleotide sequence of hIL2Rα was obtained from GenBank (Accession number X01057.1). |
| EMBL Accession number: | X01057.1, view at EMBL, GenBank, DDBJ LT727001.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 25/04/2006 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: KpnI, PstI and SacI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by E. Pattyn(1). (1) Department of Biochemistry, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Takebe et al., Mol. Cell. Biol. 8 (1988), 466-472 [PMID: 2827008] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pMET7hIL-2Ralpha (LMBP 5280) is available at BCCM/GeneCorner. This plasmid was deposited by E. Pattyn. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.