Last data update: 24 January 2024 16:39 CET
Plasmid name: pMG1-hEFEMP2-MAPPIT (LMBP 7069)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human EGF containing fibulin extracellular matrix protein 2 cDNA (EFEMP2, FBLN4, UPH1, GeneID 30008); fragment Human interleukin 6 cytokine family signal transducer cDNA (IL6ST, GP130, CD130, sGP130, IL-6RB, GeneID 3572); fragment Xpress epitope; N-terminal Histidine tag (His-tag); N-terminal FLAG epitope tag; N-terminal |
| Promoter: | Simian virus 40 early/human T-cell lymphotropic virus type 1 LTR hybrid promoter (SRα) Escherichia coli lac operon promoter; mutant (lacUV5) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Chloramphenicol (cam) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pMG1-MAPPIT |
| Further information: | The plasmid was constructed by cloning part of the human EFEMP2 coding sequence into the pMG1-MAPPIT vector via Gateway LR recombination This plasmid allows for Mammalian Protein-Protein Interaction Trap (MAPPIT) analysis of human EFEMP2. Other name of the plasmid is pdMG1-EFEMP2. |
| EMBL Accession number: | - |
| Latest sequence update: | 22/03/2011 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr F. Van Roy(1) (2). It was constructed by I. Kahr(1)(2). (1) UGent-VIB Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pMG1-hEFEMP2-MAPPIT (LMBP 7069) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr F. Van Roy . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.