Last data update: 24 January 2024 16:39 CET
Plasmid name: pMG2-hZYX (LMBP 8429)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p8429.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human zyxin cDNA (ZYX) Human interleukin 6 signal transducer cDNA (IL6ST, CD130, GP130, CDW130, IL-6RB, GeneID 3572); C-terminal fragment, N-terminal FLAG epitope tag; N-terminal |
| Promoter: | Simian virus 40 early/human T-cell lymphotropic virus type 1 LTR hybrid promoter (SRα) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pMG2 |
| Further information: | The plasmid was constructed by cloning the human ZYX coding sequence into the pMG2 vector. The pMG2 backbone contains aa 760-918 of the gp130 chain, of which aa 905-918 were duplicated. Other name of the plasmid is pMG2-Zyxin cl18. |
| EMBL Accession number: | - |
| Latest sequence update: | 16/05/2017 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Prof. Dr F. Van Roy(1)(2). It was constructed by Dr I. Kahr(1)(2). (1) VIB Center for Inflammation Research, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pMG2-hZYX (LMBP 8429) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr F. Van Roy. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.