Last data update: 24 January 2024 16:39 CET
Plasmid name: pMOL322 (LMBP 8043)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) Neomycin (neo; kanamycin (kan)) Tetracycline (tet) Pseudomonas ARGI; auxotrophic Pseudomonas ARGF; auxotrophic |
| Replicon: | - |
| Host range: | Escherichia coli |
| Parental clone: | pULB113 |
| Further information: | The plasmid was constructed in vivo based on natural plasmids. The plasmid belongs to the IncP/P-1 incompatibility family. pMOL322 contains prophage Mu3A (mini-Mu). |
| EMBL Accession number: | - |
| Latest sequence update: | 18/03/2013 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by M. Mergeay(1) and A. Provoost(1). (1) SCK.CEN, Mol, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Faelen et al., Cold Spring Harb. Symp. Quant. Biol. 43 (1979), 1169-1177 [PMID: 158471] [DOI: 10.1101/SQB.1979.043.01.132] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MXR (LMBP 13358) |
| Host reference: | Faelen et al., Cold Spring Harb. Symp. Quant. Biol. 43 (1979), 1169-1177 [PMID: 158471] [DOI: 10.1101/SQB.1979.043.01.132] |
| Helper plasmid: | no resident plasmids or phages |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) + kanamycin (50 μg/ml) + tetracycline (10 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | SCK CM0299 |
Refer in your Materials and Methods: |
pMOL322 (LMBP 8043) is available at BCCM/GeneCorner. This plasmid was deposited by M. Mergeay and A. Provoost. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.