Last data update: 24 January 2024 16:39 CET
Plasmid name: pMSCV-VSV-hPIDD1-FLAG (LMBP 7791)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human p53-induced death domain protein 1 cDNA (PIDD1, LRDD, PIDD) Vesicular stomatitis virus glycoprotein (VSV-G) epitope; N-terminal FLAG epitope tag; C-terminal |
| Promoter: | Mouse stem cell PCMV virus long terminal repeat (5' MSCV-PCMV LTR) Mouse phosphoglycerate kinase 1 promoter (PGK1) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Mouse stem cell PCMV virus long terminal repeat (3' MSCV-PCMV LTR) |
| Selection marker: | Puromycin (puro) Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli; preferably recombination-deficient strains Mammalian cells |
| Parental clone: | pMSCVpuro |
| Further information: | The plasmid was constructed by PCR amplifying the 5' region of human PPID1 from IMAGE clone 3908102 and the 3' region from IMAGE clone 4852968. A 5' VSV-tag and a 3' FLAG epitope were added during PCR amplification. The human PPID1 coding sequence was fused, and cloned into the pMSCVpuro vector. The plasmid contains the mouse stem cell PCMV (PCC4-cell-passaged myeloproliferative sarcoma virus) virus long terminal repeat. The LTR drives high-level constitutive expression of human PIDD1 in stem cells and other mammalian cell lines. The plasmid contains also the extended retroviral packaging signal, Ψ+, which promotes high-titer production of replication-incompetent infectious viral particles after introduction into a packaging cell line. Other name of the plasmid is pMSCV-PIDDisof1-Flag. |
| EMBL Accession number: | - |
| Latest sequence update: | 28/06/2018 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr P Schneider(1). (1) Department of Biochemistry, University of Lausanne, Epalinges, Switzerland |
| Plasmid reference: | Tinel and Tschopp, Science 304 (2004), 843-846 [PMID: 15073321] [DOI: 10.1126/science.1095432] |
| Related plasmid reference: | Cuenin et al., Oncogene 27 (2008), 387-396 [PMID: 17637755] [DOI: 10.1038/sj.onc.1210635] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pMSCV-VSV-hPIDD1-FLAG (LMBP 7791) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr P Schneider and was published in Tinel and Tschopp, 2004. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.