Last data update: 24 January 2024 16:39 CET
Plasmid name: pMV306DIhsp+LuxG13 (LMBP 11308)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p11308.gb
(View with Genome Compiler) p11308.txt p11308.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Photorhabdus luminescens luciferase alpha subunit DNA (luxA) Photorhabdus luminescens luciferase beta subunit DNA (luxB) Photorhabdus luminescens fatty acid reductase DNA (luxC) Photorhabdus luminescens acyl transferase DNA (luxD) Photorhabdus luminescens acyl-protein synthetase DNA (luxE) |
| Promoter: | Mycobacterium bovis molecular chaperone GroEL promoter (groEL2) Mycobacterium marinum G13 promoter (G13) |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli rrnB operon T1 terminator |
| Selection marker: | Neomycin (neo; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Mycobacterium |
| Parental clone: | pMV306hsp+LuxG13 |
| Further information: | The plasmid was constructed by removing the integrase coding sequence from pMV306hsp+LuxG13. The luxA coding sequence is preceded by a groEL2 promoter. The luxC coding sequence is preceded by a G13 promoter, resulting in a 6-fold increase in the luminescence activity compared to a construct with only the groEL2 promoter. Other name of the plasmid is pMV306hsp+LuxG13_without_integrase. |
| EMBL Accession number: | - |
| Latest sequence update: | 03/11/2020 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BglII/XhoI, BstXI, HindIII, NotI and SacII. The BstXI site on position 5836 could not be experimentally confirmed. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr N. Callewaert(1)(2). It was constructed by Dr K. Borgers(1)(2). (1) VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium (2) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Borgers et al., mSystems 5 (2020), e00180-20 [PMID: 32788404] [DOI: 10.1128/mSystems.00180-20] |
| Related plasmid reference: | Andreu et al., PLoS One. 5 (2010), e10777 [PMID: 20520722] [DOI: 10.1371/journal.pone.0010777] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αT1R |
| Host reference: | - |
| Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pMV306DIhsp+LuxG13 (LMBP 11308) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr N. Callewaert and was published in Borgers et al., 2020. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.