Last data update: 24 January 2024 16:39 CET
Plasmid name: pMaSVTHIL62 (LMBP 2332)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p2332.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Simian virus 40 (SV40) gene encoding large T-antigen |
| Promoter: | Human interleukin 6 promoter (IL6) |
| Ribosome binding site: |
- |
| Terminator: | Phage fd terminator Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pMaSVT2; pGEMIL6gIL2f1 |
| Further information: | The plasmid was constructed by replacing the EcoRI-XhoI fragment from pMaSVT2 (containing the SV40-E promoter) by the EcoRI-XhoI fragment from pGEMIL6gIL2f1. Hence, the SV40 large T-antigen is placed under control of the human interleukin 6 promoter. This plasmid differs from pMaSVTHIL61 in the length of the 5' flanking region of the hIL6 promoter. pMaSVTHIL62 has 12 nucleotides more and 3 BamHI sites, instead of 2. After mutagenesis, use mutS strains for primary transformation (e.g. sup(-) strain WK6mutS, sup(+) strain BMH71-18mutS); for segregation of possible mutants: sup(-) strains (e.g. WK6). Name of the phasmid mentioned in Vanhoenacker et al. (1994) is pMaHIL6SVT. The nucleotide sequence of the genomic hIL6 DNA corresponds with the EMBL Nucleotide Sequence Database accession number AC073072.11. However, the C nucleotide at promoter position -174 (polymorphism mutant) was changed into a G nucleotide (wild type). |
| EMBL Accession number: | AC073072.11, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 30/04/2004 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr P. Vanhoenacker(1) and Prof. Dr G. Haegeman(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Vanhoenacker et al., Eur. Cytok. Netw. 5 (1994), 283-291 [PMID: 7948766] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pMaSVTHIL62 (LMBP 2332) is available at BCCM/GeneCorner. This plasmid was deposited by Dr P. Vanhoenacker and Prof. Dr G. Haegeman and was published in Vanhoenacker et al., 1994. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.