Last data update: 24 January 2024 16:39 CET
Plasmid name: pMaT4HTNFm32W146K (LMBP 2646)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p2646.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human tumor necrosis factor cDNA (TNF); mutated mature sequence |
| Promoter: | Phage T4 gene 32 promoter (T4g32) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the phage T4 gene 32 (T4g32) |
| Terminator: | Phage fd terminator Escherichia coli tryptophan operon terminator (trp); synthetic sequence |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pMcT4HTNFm32W |
| Further information: | The plasmid was constructed by site-specific mutagenesis of pMcT4HTNFm32W, where AA146 (GAG) of mature hTNF encoding glutamic acid (E) was replaced by AAG, encoding lysine (K). After mutagenesis, use mutS strains for primary transformation (e.g. sup(-) strain WK6mutS, sup(+) strain BMH71-18mutS); for segregation of possible mutants: sup(-) strains (e.g. WK6). The nucleotide sequence of the wt hTNF cDNA corresponds with the EMBL Nucleotide Sequence Database accession number X01394.1. Other name of the plasmid is pMaT4HTNFTm32W146K |
| EMBL Accession number: | X01394.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 14/12/1992 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Van Ostade et al., EMBO J. 10 (1991), 827-836 [PMID: 2009860] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 SURE |
| Host reference: | Greener, Strategies 3 (1990), 5-6 |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pMaT4HTNFm32W146K (LMBP 2646) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert and was published in Van Ostade et al., 1991. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.