Last data update: 24 January 2024 16:39 CET
Plasmid name: pMcT4HTNFm86F (LMBP 2602)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p2602.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human tumor necrosis factor cDNA (TNF); mutated mature sequence |
| Promoter: | Phage T4 gene 32 promoter (T4g32) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the phage T4 gene 32 (T4g32) |
| Terminator: | Phage fd terminator Escherichia coli tryptophan operon terminator (trp); synthetic sequence |
| Selection marker: | Chloramphenicol (cam) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pMa-T4-hTNF-TA |
| Further information: | The plasmid was constructed by site-specific mutagenesis of pMa-T4-hTNF-TA. As a result, codon 86 (TCC) of mature hTNF encoding serine (S) was replaced by TTC encoding phenylalanine (F). After mutagenesis, use mutS strains for primary transformation (e.g. sup(-) strain WK6mutS, sup(+) strain BMH71-18mutS); for segregation of possible mutants: sup(-) strains (e.g. WK6). The nucleotide sequence of the wt hTNF cDNA corresponds with the EMBL Nucleotide Sequence Database accession number X01394.1. Other name of the plasmid is pMc-T4HTNFTm86F. |
| EMBL Accession number: | X01394.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 10/12/1991 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Van Ostade et al., EMBO J. 10 (1991), 827-836 [PMID: 2009860] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 SURE |
| Host reference: | Greener, Strategies 3 (1990), 5-6 |
| Cultivation medium: | LB-Lennox + chloramphenicol (25 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pMcT4HTNFm86F (LMBP 2602) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert and was published in Van Ostade et al., 1991. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.