Last data update: 24 January 2024 16:39 CET
Plasmid name: pMxCAThTNFR55i (LMBP 3905)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p3905.gb
(View with Genome Compiler) p3905.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Chloramphenicol acetyltransferase gene (cat) Human tumor necrosis factor receptor p55 cDNA (TNFR55, TNFR1, TNFRSF1A); intracellular domain |
| Promoter: | Mouse Mx promoter Escherichia coli lac operon promoter Phage SP6 promoter |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Mammalian cells |
| Parental clone: | pMxCAT; pMxhTNFR55i |
| Further information: | This plasmid was constructed as follows: the intracellular domain of the hTNFR55 cDNA was recovered from pMxhTNFR55i and introduced into pMxCAT, in frame at the 3' end of the chloramphenicol acetyltransferase coding sequence and separated by an oligonucleotide coding for a flexible spacer peptide Gly-Gly-Ser-Gly. When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727280.1. |
| EMBL Accession number: | LT727280.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 13/01/1999 |
| Sequence detail: | Nucleotide sequence around the fusion between the chloramphenicol acetyltransferase gene and the intracellular domain of the human tumor necrosis factor receptor p55 cDNA:
| |--> human TNF receptor p55
cat --> | | |--> intracellular domain
| spacer |201 |204
5' … .GGC.GGG.GCG.GGC.GGA.TCC.GGC.ATG.GGT.TTA.ATG.TAT.CGC.TAC. … 3'
Gly Gly Ala Gly Gly Ser Gly Met Gly Leu Met Gly Leu Tyr |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, BglI, EcoNI, HindIII and ScaI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr G. Haegeman(1) and constructed by Dr V. Vandevoorde(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Vandevoorde et al., J. Cell Biol. 137 (1997), 1627-1638 [PMID: 9199176] |
| Related plasmid reference: | Boone et al., FEBS Lett. 441 (1998), 275-280 [PMID: 9883899] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pMxCAThTNFR55i (LMBP 3905) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr G. Haegeman and constructed by Dr V. Vandevoorde and was published in Vandevoorde et al., 1997. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.