Last data update: 24 January 2024 16:39 CET
Plasmid name: pNIMXT-rtTA (LMBP 10508)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p10508.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Reverse tetracycline-responsive transcriptional activator (rtTA); codon optimised for C. albicans (CartTA) |
| Promoter: | Candida albicans actin promoter (ACT1) Candida albicans glyceraldehyde-3-phosphate dehydrogenase promoter (TDH3) Candida albicans alcohol dehydrogenase 1 promoter (ADH1) Phage T3 promoter Phage T7 gene 10 promoter (T7g10) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Candida albicans actin terminator (ACT1) Candida albicans ubiquinol cytochrome-c reductase subunit 7 terminator (QCR7) |
| Selection marker: | Ampicillin (amp) Nourseothricin (ntc) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli Candida albicans; integrative |
| Parental clone: | pNIMX |
| Further information: | The plasmid was constructed by replacing the C. albicans ACT1 terminator and URA3 terminator between the cartTA and C. albicans SAT1 coding sequence in pNIMX with the bidirectional C. albicans QCR7 terminator. The plasmid is intended for chromosomal integration of the nourseothricin cassette into the C. albicans genome via recombination with the ADH1 sequences. Integration allows for tetracycline/doxycycline regulated expression of genes via a Tet-On transactivator, under the control of the C. albicans TDH3 promoter. rtTA, a fusion of the E. coli derived reverse Tet repressor and the S. cerevisiae GAL4 activation domain, is codon optimised for C. albicans. CaSAT1, a fusion of C. albicans ACT1 including the intron and E. coli SAT1, confers resistance to nourseothricin. The nucleotide sequence of the plasmid corresponds with the EMBL Nucleotide Sequence Database accession number KX766188.1. |
| EMBL Accession number: | KX766188.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 05/12/2017 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Dr K. Ganesan(1). It was constructed by Dr S. Bijlani(1). (1) CSIR Institute of Microbial Technology, Chandigarh, India |
| Plasmid reference: | Bijlani et al., Curr. Genet. 64 (2018), 303-316 [PMID: 28597304] [DOI: 10.1007/s00294-017-0720-9] |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pNIMXT-rtTA (LMBP 10508) is available at BCCM/GeneCorner. The plasmid was deposited by Dr K. Ganesan and was published in Bijlani et al., 2018. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.