Last data update: 24 January 2024 16:39 CET
Plasmid name: pOSV803 (LMBP 11371)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p11371.gb
(View with Genome Compiler) p11371.txt p11371.pdf |
| Sequence analysis results Genecorner: |
NGS: a08-gc-dec2019.fasta |
| Cloned DNA: |
Streptomyces ambofaciens pSAM2 integrase cDNA (int, GeneID 24955078) Acropora millepora blue chromoprotein cDNA (amilCP) |
| Promoter: | Escherichia coli NADH:quinone oxidoreductase II promoter (ndh) Escherichia coli hybrid tryptophan/lacUV5 promoter (trc) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | Escherichia coli rrnB operon T1 terminator Phage T7 early transcription terminator (T7Te) |
| Selection marker: | Apramycin (apr) |
| Replicon: | Escherichia coli plasmid p15A origin Broad-host-range plasmid RK2/RP4 origin of transfer (oriT) |
| Copy number: | Low copy number |
| Host range: | Escherichia coli Streptomyces sp.; integrative |
| Parental clone: | pOSV800 |
| Further information: | The plasmid was constructed by replacing the phage phiBT1 integration cassette of pOSV800 by the pSAM2 integration cassette, after removal of the BamHI and KpnI sites from the pSAM2 integration cassette by site-directed mutagenesis. This is a modular integrative cloning vector for the assembly of DNA and expression in Streptomyces species via integration at the pSAM2 attP site in the Streptomyces chromosome. The nucleotide sequence of the pSAM2 features corresponds with the Genbank accession number AJ005260.1. |
| EMBL Accession number: | AJ005260.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 21/05/2019 |
| Authenticity test: | This plasmid has been fully sequenced by BCCM/GeneCorner. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Dr S. Lautru(1). It was constructed by C. Aubry(1). (1) Institut de Biologie Intégrative de la Cellule (I2BC), University of Paris-Sud, Orsay, France |
| Plasmid reference: | Aubry et al., Appl. Environ. Microbiol. 85 (2019), e00485-19 [PMID: 31175189] [DOI: 10.1128/AEM.00485-19] |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + apramycin (50 µg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | Addgene 126596 |
Refer in your Materials and Methods: |
pOSV803 (LMBP 11371) is available at BCCM/GeneCorner. The plasmid was deposited by Dr S. Lautru and was published in Aubry et al., 2019. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.