Last data update: 24 January 2024 16:39 CET
Plasmid name: pOSV805 (LMBP 11373)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p11373.gb |
| Sequence analysis results Genecorner: |
NGS: a10-gc-dec2019.fasta |
| Cloned DNA: |
Streptomyces phage phiBT1 integrase cDNA (int, GeneID 1258910) Acropora millepora blue chromoprotein cDNA (amilCP) |
| Promoter: | Escherichia coli NADH:quinone oxidoreductase II promoter (ndh) |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli rrnB operon T1 terminator Phage T7 early transcription terminator (T7Te) |
| Selection marker: | Hygromycin (hyg) |
| Replicon: | Escherichia coli plasmid p15A origin Broad-host-range plasmid RK2/RP4 origin of transfer (oriT) |
| Copy number: | Low copy number |
| Host range: | Escherichia coli Streptomyces sp.; integrative |
| Parental clone: | pOSV801 |
| Further information: | The plasmid was constructed by replacing the aac(3)IV apramycin resistance gene of pOSV801 by the aph(7") hygromycin resistance gene. This is a cloning vector for the assembly of DNA and integration at the phiBT1 attP site in the Streptomyces chromosome. |
| EMBL Accession number: | - |
| Latest sequence update: | 21/05/2019 |
| Authenticity test: | This plasmid has been fully sequenced. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Dr S. Lautru(1). It was constructed by C. Aubry(1). (1) Institut de Biologie Intégrative de la Cellule (I2BC), University of Paris-Sud, Orsay, France |
| Plasmid reference: | Aubry et al., Appl. Environ. Microbiol. 85 (2019), e00485-19 [PMID: 31175189] [DOI: 10.1128/AEM.00485-19] |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + hygromycin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | Addgene 126598 |
Refer in your Materials and Methods: |
pOSV805 (LMBP 11373) is available at BCCM/GeneCorner. The plasmid was deposited by Dr S. Lautru and was published in Aubry et al., 2019. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.