Last data update: 24 January 2024 16:39 CET
Plasmid name: pPAS2 (LMBP 2082)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | ppas2.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Escherichia coli papA gene (papA); mutant 7B |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | Phage fd terminator |
| Selection marker: | Chloramphenicol (cam) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pPAS1 |
| Further information: | This plasmid was constructed by site-specific mutagenesis (Eckstein method) of the papA gene; a BamHI site is created at AA 7 of mature papA. The plasmid contains the mutated, promoterless papA gene. After mutagenesis, use mutS strains for primary transformation (e.g. sup(-) strain WK6mutS, sup(+) strain BMH71-18mutS); for segregation of possible mutants: sup(-) strains (e.g. WK6). Other name of the plasmid is pMc58PAPA7B. |
| EMBL Accession number: | - |
| Latest sequence update: | 04/06/1993 |
| Sequence detail: | Mutation of the E. coli papA gene:
signal sequence
ATG ATT AAG TCG GTT ATT GCC GGT GCG GTA GCT ATG GCA GTG GTG TCT
MET Ile Lys Ser Val Ile Ala Gly Ala Val Ala Met Ala Val Val Ser
| mature papA
| 1 2 3 4 5 6 7 8 9 10
TTT GGT GTA AAT AAT GCT GCT CCA ACT ATT CCA CAG GGG GAT CCT AAA
Phe Gly Val Asn Asn Ala Ala Pro Thr Ile Pro Gln Gly Asp Asn Lys
BamHI
E. coli papA gene wt :
signal sequence
ATG ATT AAG TCG GTT ATT GCC GGT GCG GTA GCT ATG GCA GTG GTG TCT
MET Ile Lys Ser Val Ile Ala Gly Ala Val Ala Met Ala Val Val Ser
| mature papA
| 1 2 3 4 5 6 7 8 9 10
TTT GGT GTA AAT AAT GCT GCT CCA ACT ATT CCA CAG GGG CAG GGT AAA
Phe Gly Val Asn Asn Ala Ala Pro Thr Ile Pro Gln Gly Gln Gly Lys |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr L. Steidler(1) (2) and Prof. Dr E. Remaut(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Steidler et al., J. Bacteriol. 175 (1993), 7639-7643 [PMID: 7902348] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + chloramphenicol (25 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPAS2 (LMBP 2082) is available at BCCM/GeneCorner. This plasmid was deposited by Dr L. Steidler and Prof. Dr E. Remaut and was published in Steidler et al., 1993. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.