Last data update: 24 January 2024 16:39 CET
Plasmid name: pPB-TRE-TurboID-T2A-NSP7_PGK-BlastR (LMBP 13901)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nonstructural protein 7 cDNA (NSP7) V5 epitope; N-terminal TurboID tag; N-terminal Thosea asigna virus 2A peptide (T2A) |
| Promoter: | Tetracycline-responsive promoter element (TRE) Mouse phosphoglycerate kinase 1 promoter (PGK1) Phage T7 gene 10 promoter (T7g10) Phage T3 promoter Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) Bovine growth hormone polyadenylation signal (BGH polyA) |
| Selection marker: | Blasticidin (bsd) Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli Mammalian cells |
| EMBL Accession number: | - |
| Latest sequence update: | 07/11/2023 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by T. Van de Steene(1)(2) and Prof. Dr S. Eyckerman(1)(2). (1) VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium (2) Department of Biomolecular Medicine, Ghent University, Ghent, Belgium |
| Plasmid reference: | Delhaye et al., Cell Rep. Methods (2023), submitted [DOI: 10.1101/2023.11.03.565112] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH10B |
| Host reference: | Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Related host reference: | Durfee et al., J. Bacteriol. 190 (2008), 2597-2606 [PMID: 18245285] [DOI: 10.1128/JB.01695-07] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPB-TRE-TurboID-T2A-NSP7_PGK-BlastR (LMBP 13901) is available at BCCM/GeneCorner. This plasmid was deposited by T. Van de Steene and Prof. Dr S. Eyckerman and was published in Delhaye et al., 2023. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.