Last data update: 24 January 2024 16:39 CET
Plasmid name: pPICZ-E-MALT1Cd180::GyrB-Myc-His (LMBP 5745)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human MALT1 paracaspase cDNA (MALT1, MLT, PCASP1, GeneID 10892); fragment Escherichia coli DNA gyrase subunit B (gyrB); fragment E-tag; N-terminal Myc epitope; C-terminal Histidine tag (His-tag); C-terminal |
| Promoter: | Pichia pastoris alcohol oxidase 1 promoter (AOX1) Saccharomyces cerevisiae translation elongation factor 1α promoter (TEF1α) Synthetic prokaryotic EM7 promoter |
| Ribosome binding site: |
- |
| Terminator: | Pichia pastoris alcohol oxidase 1 terminator (AOX1) Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1) |
| Selection marker: | Bleomycin (bleo; zeomycin (zeo; Zeocin); phleomycin (phleo)) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Pichia pastoris; integrative |
| Parental clone: | pPICZA; pCAGGS-E-MALT1Cd180::GyrB |
| Further information: | The plasmid was constructed by PCR amplifying the E-tagged human MALT1Cd180::GyrB fragment from pCAGGS-E-MALT1Cd180::GyrB and ligating it as a KpnI/ApaI fragment into the pPICZA vector. The plasmid was created for production of recombinant human MALT1 in Pichia pastoris for in vitro studies. It encodes for residues 334-664 of human MALT1 fused to the E. coli GyraseB dimerization domain. The nucleotide sequence of the human MALT1 coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number AF130356.2. Other name of the plasmid is pPICZ-MALT1C d180 Gyr B |
| EMBL Accession number: | AF130356.2, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 09/04/2009 |
| Sequence detail: | Primers used to amplify the E-tagged Malt1Cd180::GyrB fragment: ApaI-GyrB-R: 5' ggcGGGCCCGCCTTCATAGTGGAAGTGGTCTT KpnI-pCAGGS-E-F: 5' gaattGGTACCaattccaccatgggtgcgcc |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr J. Staal(1) (2) and Prof. Dr R. Beyaert(1) (2). (1) UGent-VIB Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + zeocin (25 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPICZ-E-MALT1Cd180::GyrB-Myc-His (LMBP 5745) is available at BCCM/GeneCorner. This plasmid was deposited by Dr J. Staal and Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.