Last data update: 24 January 2024 16:39 CET
Plasmid name: pPICZA-AGA1 (LMBP 8501)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Saccharomyces cerevisiae Aga1p cDNA (AGA1, GeneID 855780) |
| Promoter: | Pichia pastoris alcohol oxidase 1 promoter (AOX1) Saccharomyces cerevisiae translation elongation factor 1α promoter (TEF1α) Synthetic prokaryotic EM7 promoter |
| Ribosome binding site: |
- |
| Terminator: | Pichia pastoris alcohol oxidase 1 terminator (AOX1) Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1) |
| Selection marker: | Bleomycin (bleo; zeomycin (zeo; Zeocin); phleomycin (phleo)) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli; preferably recombination-deficient and endonuclease A deficient strains Pichia pastoris; integrative |
| Parental clone: | pPICZA |
| Further information: | The plasmid was constructed by cloning the S. cerevisiae Aga1p coding sequence as an XhoI/ApaI fragment into the pPICZA vector. This plasmid is part of a Pichia Surface Display system, together with the pPIC9-AGA2 vector (LMBP 08502). Other names of the plasmid are pPICZA AGA1, pPICZAAGA1 and pPICZA AGA1 -XhoI/-ApaI. |
| EMBL Accession number: | - |
| Latest sequence update: | 12/07/2013 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Dr K. De Schutter(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Jacobs et al., Biotechnol. Lett. 30 (2008), 2173-2181 [PMID: 18679585] [DOI: 10.1007/s10529-008-9807-1] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + zeocin (25 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPICZA-AGA1 (LMBP 8501) is available at BCCM/GeneCorner. The plasmid was deposited by Dr K. De Schutter and was published in Jacobs et al., 2008. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.