Last data update: 24 January 2024 16:39 CET
Plasmid name: pPICZBBAX (LMBP 6635)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p6635.gb
(View with Genome Compiler) p6635.txt p6635.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse Bcl2-associated X cDNA (Bax, BAX); α variant Myc epitope; C-terminal Histidine tag (His-tag); C-terminal |
| Promoter: | Pichia pastoris alcohol oxidase 1 promoter (AOX1) Saccharomyces cerevisiae translation elongation factor 1α promoter (TEF1α) Synthetic prokaryotic EM7 promoter |
| Ribosome binding site: |
- |
| Terminator: | Pichia pastoris alcohol oxidase 1 terminator (AOX1) Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1) |
| Selection marker: | Bleomycin (bleo; zeomycin (zeo; Zeocin); phleomycin (phleo)) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli; preferably recombination-deficient and endonuclease A deficient strains Pichia pastoris; integrative |
| Parental clone: | pUC19B; pPICZB |
| Further information: | The plasmid was created by isolating the mBax cDNA from pUC19B as a HindIII (Klenow blunted)/EcoRI fragment and cloning it into the NotI (Klenow blunted)/EcoRI-opened pPICZB vector. The C-terminal Myc epitope and histidine tag are not expressed. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726836.1. The nucleotide sequence of the mBax cDNA corresponds with the EMBL Nucleotide Sequence Database accession number L22472.1. |
| EMBL Accession number: | L22472.1, view at EMBL, GenBank, DDBJ LT726836.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 16/03/2010 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, EcoRI/SphI, EcoRV and Alw44I. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr. P. Tiels(1) (2) and Prof. Dr N. Callewaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Martinet et al., Biotechnol. Lett. 21 (1999), 821-829 |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + zeocin (25 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPICZBBAX (LMBP 6635) is available at BCCM/GeneCorner. This plasmid was deposited by Dr. P. Tiels and Prof. Dr N. Callewaert and was published in Martinet et al., 1999. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.