Last data update: 24 January 2024 16:39 CET
Plasmid name: pPLSTi722 (LMBP 2030)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p2030.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Satellite tobacco necrosis virus (STNV); with insertion |
| Promoter: | Phage λ major leftward promoter (λ PL) |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli; use strains with a cI function |
| Parental clone: | pPLST10 |
| Further information: | This plasmid is derived from pPLST10; into the RsaI site (nucleotide position 722 of STNV, partial digestion) the EcoRI-NcoI linker (AGAATTCCCATGGA) was inserted. Other names of the plasmid are pPLSTNVE720 and pSTPU1l41. |
| EMBL Accession number: | - |
| Latest sequence update: | 22/09/1989 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr P. Ameloot(1) and Prof. Dr W. Fiers(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061(λ) |
| Host reference: | Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329] |
| Related host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPLSTi722 (LMBP 2030) is available at BCCM/GeneCorner. This plasmid was deposited by Dr P. Ameloot and Prof. Dr W. Fiers. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.