Last data update: 24 January 2024 16:39 CET
Plasmid name: pPLa10 (LMBP 906)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | ppla10.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | Phage λ major leftward promoter (λ PL) |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) Kanamycin (kan) |
| Replicon: | Escherichia coli plasmid ColE1 origin |
| Host range: | Escherichia coli; use strains with a cI function, cIts for PL controlled expression |
| Parental clone: | pPLa2311 |
| Further information: | The PstI site in the ampicillin resistance gene of pPLa2311 was replaced by a SmaI site, resulting in a modified but active β-lactamase. There is another SmaI site in the kanamycin resistance gene. |
| EMBL Accession number: | - |
| Latest sequence update: | 06/07/1987 |
| Sequence detail: | Nucleotide sequence around the SmaI site in the ampicillin gene:
5' ACC.ACG.ATG.CCC.CGG.GCA.ATG 3'
SmaI
Punctuation indicates reading frame in the modified active beta-lactamase. |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr E. Remaut(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 K514(λ) |
| Host reference: | Zabeau et al., EMBO J. 1 (1982), 1217-1224 [PMID: 6327257] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) + kanamycin (50 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPLa10 (LMBP 906) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.