Last data update: 24 January 2024 16:39 CET
Plasmid name: pPLa231 (LMBP 217)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
ppla231.gb
(View with Genome Compiler) ppla231.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | Phage λ major leftward promoter (λ PL) |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) Kanamycin (kan) |
| Replicon: | Escherichia coli plasmid ColE1 origin |
| Host range: | Escherichia coli; use strains with a cI function, cIts for PL controlled expression |
| Parental clone: | pPLa23; pMK20 |
| Further information: | The plasmid is designed for expression of fragments containing a ribosome binding site functional in E. coli. The plasmid differs from pPLa2311 in the orientation of the kanamycin resistance gene, which is here anticlockwise. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727627.1. |
| EMBL Accession number: | LT727627.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 23/03/1992 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: HindIII, PvuI and RsaI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr E. Remaut(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 M5219 |
| Host reference: | Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) + kanamycin (50 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPLa231 (LMBP 217) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.