Last data update: 24 January 2024 16:39 CET
Plasmid name: pPLaA2 (LMBP 291)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
pplaa2.gb
(View with Genome Compiler) pplaa2.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Phage MS2 assembly protein gene Phage MS2 coat protein gene; fragment |
| Promoter: | Phage λ major leftward promoter (λ PL) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the phage MS2 assembly protein gene Ribosome binding site (RBS) of the phage MS2 coat protein gene |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) Kanamycin (kan) |
| Replicon: | Escherichia coli plasmid ColE1 origin |
| Host range: | Escherichia coli; use strains with a cI function, cIts for PL controlled expression |
| Parental clone: | pPLa2311; pMS27 |
| Further information: | The EcoRI fragment of pMS27, containing the complete MS2 assembly protein coding sequence and the coding information for the first 97 amino acids of the MS2 coat protein, was cloned into the EcoRI site of pPLa2311 in the sense orientation relative to the λ PL promoter. Other name of the plasmid is pSRKM2. |
| EMBL Accession number: | - |
| Latest sequence update: | 25/03/1992 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: AvaI, EcoRI, HindIII, PvuI, PvuI/StyI and StyI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr E. Remaut(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Remaut et al., EMBO J. 1 (1982), 205-209 [PMID: 16453413] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 M5219 |
| Host reference: | Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) + kanamycin (50 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPLaA2 (LMBP 291) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut and was published in Remaut et al., 1982. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.