Last data update: 24 January 2024 16:39 CET
Plasmid name: pPLc236 (LMBP 379)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
pplc236.gb
(View with Genome Compiler) pplc236.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | Phage λ major leftward promoter (λ PL) |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli; use strains with a cI function, cIts for PL controlled expression |
| Parental clone: | pPLc23 |
| Further information: | Designed for λ PL promoter-regulated expression of fragments containing a ribosome binding site functional in Escherichia coli. Expression sites: BalI, BamHI, BspMII, EcoRI and HindIII. BamHI, BspMII and HindIII are not unique. The BalI site in the polylinker downstream from the λ PL promoter can be used as a unique expression site since the other BalI site cuts only in dcm-negative strains. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727393.1. Other name of the plasmid is pST236. |
| EMBL Accession number: | LT727393.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 30/01/1989 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: HindII, PvuI and RsaI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr E. Remaut(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 M5219 |
| Host reference: | Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPLc236 (LMBP 379) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut and was published in Remaut et al., 1981. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.