Last data update: 24 January 2024 16:39 CET
Plasmid name: pPLc2408 (LMBP 1311)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | pplc2408.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Phage MS2 polymerase gene; first 98 codons |
| Promoter: | Phage λ major leftward promoter (λ PL) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the phage MS2 polymerase gene |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli; use strains with a cI function, cIts for PL controlled expression |
| Parental clone: | pPLc24 |
| Further information: | Designed for expression of fused proteins with MS2 polymerase. This plasmid is very similar to pPLc24, except at the position of the insertion sites. |
| EMBL Accession number: | - |
| Latest sequence update: | 31/01/1989 |
| Sequence detail: | Nucleotide sequence around the insertion sites:
*
5' TCA.TGG.GAT.CTG.AAT.TCC.GGG.GAT.CCG.TCG.ACC.TGC.AGC.CAA.GCT.TGG 3'
EcoRI BamHI SalI PstI HindIII
*: 98th codon of MS2 polymerase.
Punctuation indicates reading frame. |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr E. Remaut(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061(λ) |
| Host reference: | Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329] |
| Related host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Cultivation medium: | LB-Lennox |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPLc2408 (LMBP 1311) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.