Last data update: 24 January 2024 16:39 CET
Plasmid name: pPLc245 (LMBP 682)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
pplc245.gb
(View with Genome Compiler) pplc245.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | Phage λ major leftward promoter (λ PL) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the phage MS2 polymerase gene |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli; use strains with a cI function, cIts for PL controlled expression |
| Parental clone: | pPLc24; pPLc2819; pPLc28 |
| Further information: | Designed for the expression of nonfused proteins after SalI digestion and e.g. S1 nuclease treatment of the 5' sticky ends, making the ATG start codon on the plasmid accessible for blunt-end ligation. XbaI, PstI and HindIII cannot be used for expression: the first XbaI site contains a termination codon in phase with ATG. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726843.1. |
| EMBL Accession number: | LT726843.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 02/10/1992 |
| Sequence detail: | See page 2.
Nucleotide sequence at the Shine-Dalgarno (SD) of the MS2 polymerase ribosome binding site:
5' CATGAGGATTACCC ATG.TCG.ACC.CTC.TAG.AGG.CTG.CAG.CCT.CTA.GAG.CCA.AGC.TTG 3'
------ *
SD SalI XbaI PstI XbaI HindIII
*: Termination codon.
Punctuation indicates reading frame. |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: HindII, PvuI, RsaI and TaqI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr E. Remaut(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Remaut et al., Nucleic Acids Res. 11 (1983), 4677-4688 [PMID: 6308555] |
| Related plasmid reference: | Nishihara et al., Microbiol. Immunol. 50 (2006), 61-66 [PMID: 16428875] Nishihara et al., J. Gen. Virol. 83 (2002), 2601-2606 [PMID: 12237444] [DOI: 10.1099/0022-1317-83-10-2601] Nishihara et al., Genes Cells 9 (2004), 877-889 [PMID: 15461660] [DOI: 10.1111/j.1365-2443.2004.00778.x] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 ΔH1Δtrp |
| Host reference: | Bernard et al., Gene 5 (1979), 59-76 [PMID: 372049] |
| Related host reference: | Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPLc245 (LMBP 682) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut and was published in Remaut et al., 1983. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.