Last data update: 24 January 2024 16:39 CET
Plasmid name: pPLc2820BHIL2X (LMBP 970)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p970.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human interleukin 2 cDNA (IL2); partial mature sequence (first 58 codons) Ampicillin resistance gene (amp); signal sequence |
| Promoter: | Escherichia coli β-lactamase promoter (amp) Phage λ major leftward promoter (λ PL) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli ampicillin resistance gene (amp) |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli; use strains with a cI function |
| Parental clone: | pAT153HIL2B; pPLc2820BAH3 |
| Further information: | pPLc2820BHIL2X can be used for the study of secretion of the amino terminal part of the human IL2 gene. The human IL2 fragment has a correct fusion with the signal sequence of the ampicillin resistance gene. The nucleotide sequence of the human IL2 corresponds with the EMBL Nucleotide Sequence Database accession number X01586.1), except for the first codon of the mature sequence (GCA) that is GCG. Other name of the plasmid is pPLc2820betaNILX. |
| EMBL Accession number: | X01586.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 02/02/1987 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr E. Remaut(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 K514(λ) |
| Host reference: | Zabeau et al., EMBO J. 1 (1982), 1217-1224 [PMID: 6327257] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPLc2820BHIL2X (LMBP 970) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.