Last data update: 24 January 2024 16:39 CET
Plasmid name: pPLc2820BS (LMBP 836)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p836.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Ampicillin resistance gene (amp); signal sequence |
| Promoter: | Escherichia coli β-lactamase promoter (amp) Phage λ major leftward promoter (λ PL) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli ampicillin resistance gene (amp) |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli; use strains with a cI function, cIts for PL controlled expression |
| Parental clone: | pPLc2820B |
| Further information: | The SalI site can be used for expression of proteins that are fused close to the amp signal protein. XbaI and HindIII cannot be used for expression of fused proteins: the XbaI site contains a termination codon in phase. HindII site is not an unique site. The amp promoter is not controllable. Other name of the plasmid is pPLc28-20ß279S. |
| EMBL Accession number: | - |
| Latest sequence update: | 08/06/1990 |
| Sequence detail: | Nucleotide sequence of the ampicillin gene with the polylinker:
5' ATG.AGT.ATT.CAA.CAT.TTC.CGT.GTC.GCC.CTT.ATT.CCC.TTT.TTT.GCG.
*
@
GCA.TTT.TGC.CTT.CCT.GTT.TTT.GCG.GTC.GAC.CTC.TAG AGC.CAA.GCT.T 3'
^ XbaI HindIII
SalI
HindII
*: Start of the amp coding sequence.
^: Start of the mature sequence of the amp gene.
@: Termination codon.
Punctuation indicates reading frame. |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr E. Remaut(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Leemans et al., Gene 85 (1989), 99-108 [PMID: 2695405] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 K514(λ) |
| Host reference: | Zabeau et al., EMBO J. 1 (1982), 1217-1224 [PMID: 6327257] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPLc2820BS (LMBP 836) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut and was published in Leemans et al., 1989. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.