Last data update: 24 January 2024 16:39 CET
Plasmid name: pPLc28K1 (LMBP 1479)
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Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | pplc28k1.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Escherichia coli plasmid pMB9 kil gene |
Promoter: | Phage λ major leftward promoter (λ PL) |
Ribosome binding site: |
- |
Terminator: | - |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli; use strains with a cI function, cIts for PL controlled expression |
Parental clone: | pPLc28; pMB9 |
Further information: | This plasmid contains the KIL gene from pMB9. The kil gene expression causes release of periplasmic proteins and cell death. Expression of the kil gene is dependent on supplying an anti-termination function such as phage λ's N protein. See also pPLc321K1. The nucleotide sequence of the kil gene corresponds with the EMBL Nucleotide Sequence Database accession number M13468.1. |
EMBL Accession number: | M13468.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 27/11/1991 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Dr L. Steidler(1) and Prof. Dr E. Remaut(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | Steidler et al., Biotechnol. Bioeng. 44 (1994), 1074-1082 [PMID: 18623024] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 ΔH1Δtrp |
Host reference: | Bernard et al., Gene 5 (1979), 59-76 [PMID: 372049] |
Related host reference: | Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 28°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPLc28K1 (LMBP 1479) is available at BCCM/GeneCorner. This plasmid was deposited by Dr L. Steidler and Prof. Dr E. Remaut and was published in Steidler et al., 1994. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.