Last data update: 24 January 2024 16:39 CET
Plasmid name: pPLc300 (LMBP 3658)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner core plasmid |
GeneCorner sequence: |
pplc300.gb
(View with Genome Compiler) pplc300.pdf |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
- |
Promoter: | Phage λ major leftward promoter (λ PL) |
Ribosome binding site: |
- |
Terminator: | Phage fd terminator |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
Host range: | Escherichia coli; use strains with a cI function, cIts for PL controlled expression |
Parental clone: | pPLc2820; pSE380; pMc58 |
Further information: | The plasmid was constructed by ligating four fragments: (i) the XbaI-AlwNI fragment of pMc58, containing a tandem terminator sequence of phage fd, the origin of replication of phage f1 as well as the Escherichia coli plasmid pMB1 replication origin, (ii) the AlwNI-EcoRI fragment of pPLc2820, containing the ampicillin resistance gene and the phage λ major leftward promoter (λ PL), (iii) the EcoRI-HindIII fragment of the superlinker of pSE380 and (iv) a XbaI-compatible/HindIII linker. This plasmid is designed for λ PL promoter-regulated expression of fragments containing a ribosome binding site functional in E. coli. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727368.1. |
EMBL Accession number: | LT727368.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 21/08/1997 |
Sequence detail: | linker sequence: 5' AGCTTGCCGCG 3' 3' ACGGCGCGATC 5' HindIII XbaI-compatible |
Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BglI, EcoRI, SpeI/XmnI and XbaI. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr E. Remaut(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 MC1061(λ) |
Host reference: | Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329] |
Related host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 28°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPLc300 (LMBP 3658) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.