Last data update: 24 January 2024 16:39 CET
Plasmid name: pPLc31 (LMBP 718)
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Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | pplc31.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
- |
Promoter: | Phage λ major leftward promoter (λ PL) |
Ribosome binding site: |
- |
Terminator: | - |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli; use strains with a cI function, cIts for PL controlled expression |
Parental clone: | pPLc28 |
Further information: | pPLc31 has a unique XbaI site between the λ pL promoter and the replication origin. The plasmid is designed for expression of fragments containing a ribosome binding site functional in E. coli. |
EMBL Accession number: | - |
Latest sequence update: | 29/04/1992 |
Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BglI, HaeII, HindII, PvuI and XbaI. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr E. Remaut(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | PhD thesis Eugeen Vanmechelen (1986) |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 r-m+ (λ) |
Host reference: | - |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 28°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPLc31 (LMBP 718) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut and was published in Eugeen Vanmechelen, 1986. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.