Last data update: 24 January 2024 16:39 CET
Plasmid name: pPLc32 (LMBP 719)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner core plasmid |
GeneCorner sequence: |
pplc32.gb
(View with Genome Compiler) pplc32.pdf |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
- |
Promoter: | Phage λ major leftward promoter (λ PL) |
Ribosome binding site: |
- |
Terminator: | - |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli; use strains with a cI function, cIts for PL controlled expression |
Parental clone: | pPLc28 |
Further information: | pPLc32 has a unique XbaI site between the ampicillin resistance gene and the replication origin. The plasmid is designed for expression of fragments containing a ribosome binding site functional in E. coli. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726847.1. |
EMBL Accession number: | LT726847.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 29/04/1992 |
Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, BamHI/XbaI, BglI, HaeII, HindII, PvuI and XbaI. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr E. Remaut(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 r-m+ (λ) |
Host reference: | - |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 28°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPLc32 (LMBP 719) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.