Last data update: 24 January 2024 16:39 CET
Plasmid name: pPLc321T7C10T (LMBP 1539)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p1539.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Phage T7 gene 10 (T7g10) encoding A protein (without frameshift) Phage T7 gene 10 (T7g10) encoding B protein (with frameshift) |
| Promoter: | Phage λ major leftward promoter (λ PL) Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli tryptophan operon attenuator peptide (trp) Ribosome binding site (RBS) of the phage T7 gene 10 (T7g10) |
| Terminator: | Phage T7 gene 10 terminator (T7g10) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli; use strains with a cI function, cIts for PL controlled expression |
| Parental clone: | pPLc321 |
| Further information: | This plasmid contains phage T7 gene number 10 that codes for the T7 major head protein; the protein can be expressed either from the PL promoter or from the T7 promoter (the latter dependent on the T7 RNA polymerase). The coding region has a frameshift of 2 nucleotides at the end. The protein without the frameshift is called the A protein; the protein with the frameshift is called the B protein. |
| EMBL Accession number: | - |
| Latest sequence update: | 01/10/1990 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr N. Mertens(1) and Prof. Dr E. Remaut(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 ΔH1Δtrp |
| Host reference: | Bernard et al., Gene 5 (1979), 59-76 [PMID: 372049] |
| Related host reference: | Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPLc321T7C10T (LMBP 1539) is available at BCCM/GeneCorner. This plasmid was deposited by Dr N. Mertens and Prof. Dr E. Remaut. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.