Last data update: 24 January 2024 16:39 CET
Plasmid name: pPLcHIFNB31 (LMBP 1650)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p1650.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human interferon β cDNA (IFNB, IFNB1); fragment of the mature sequence |
| Promoter: | Phage λ major leftward promoter (λ PL) |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli; use strains with a cI function, cIts for PL controlled expression |
| Parental clone: | pPLc28; pPLa-HFIF-67-1 |
| Further information: | The plasmid was constructed by inserting a fragment of the sequence coding for mature, human IFNB, provided with an EcoRI linker, as an EcoRI-BglII fragment between the EcoRI and BamHI sites of pPLc28. The coding sequence of human IFNB is not complete, it starts at the 2nd codon of the mature sequence. The nucleotide sequence of the hIFNβ cDNA corresponds with the EMBL Nucleotide Sequence Database accession number V00546.1. Other names of the plasmid are pBX32-1 and pPLcHFIF31-1. |
| EMBL Accession number: | V00546.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 07/12/1993 |
| Sequence detail: | wild type : signal sequence-->| mature human IFNB
1 2 3 4
ACA GCT CTT TCC ATG AGC TAC AAC
Thr Ala Leu Ser Met Ser Tyr Asn
pPLcHIFNB31 : 2 3 4
GAATTCCCCTCGAGG AGC TAC AAC
EcoRI Ser Tyr Asn |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr E. Remaut(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Derynck et al., Nature 287 (1980), 193-197 [PMID: 6159534] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061(λ) |
| Host reference: | Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329] |
| Related host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPLcHIFNB31 (LMBP 1650) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut and was published in Derynck et al., 1980. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.