Last data update: 24 January 2024 16:39 CET
Plasmid name: pPLcMIFNG5 (LMBP 1600)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p1600.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse interferon γ cDNA (IFNG); (slightly modified) mature sequence |
| Promoter: | Phage λ major leftward promoter (λ PL) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli tryptophan operon attenuator peptide (trp) |
| Terminator: | Phage fd terminator |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli; use strains with a cI function, cIts for PL controlled expression |
| Parental clone: | pMc58; pPLcMIFNG |
| Further information: | The plasmid was constructed by inserting a BglI-EcoRI fragment from pPLcMIFNG (containing the PL promoter and the mouse IFNG gene) in the unique EcoRI and unique BglI sites of pMc58. pPLcMFNG5 is an expression plasmid, designed for PL-controlled expression of mature mouse IFNG. The nucleotide sequence of the mouse IFNG cDNA corresponds with the EMBL Nucleotide Sequence Database accession number K00083.1, except for the slightly modified 5' side. Other name of the plasmid is pPLcM58MIFNGFdT. |
| EMBL Accession number: | K00083.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 29/05/1989 |
| Sequence detail: | signal sequence ->|-> mature mouse IFNG
Val Ser Gly Cys Tyr Cys His Gly Thr Val Ile Glu Ser Leu Glu Ser Leu
wild type: 5'...GTT.TCT.GGC|TGT.TAC.TGC.CAC.GGC.ACA.GTC.ATT.GAA.AGC.CTA.GAA.AGT.CTG...3'
MaeI
Met His Gly Thr Val Ile Glu Ser Leu Glu Ser Leu
pPLcMIFNG5: 5'...TAAAAAGGGTATCGATTCC.ATG.CAC.GGC.ACA.GTC.ATT.GAA.AGC.CTA.GAA.AGT.CTG...3'
-----------------------------------
linker MaeI |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr E. Remaut(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061(λ) |
| Host reference: | Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329] |
| Related host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPLcMIFNG5 (LMBP 1600) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.