Last data update: 24 January 2024 16:39 CET
Plasmid name: pPLcZCYA1 (LMBP 488)
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Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p488.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Phage MS2 polymerase gene; fragment Escherichia coli lac Z gene (lacZ) Escherichia coli lac Y gene (lacY) Escherichia coli lac A gene (lacA) |
Promoter: | Phage λ major leftward promoter (λ PL) |
Ribosome binding site: |
Ribosome binding site (RBS) of the phage MS2 polymerase gene |
Terminator: | - |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli; use strains with a cI function, cIts for PL controlled expression |
Parental clone: | pPLcZCYA0; pPLc24 |
Further information: | This plasmid is similar to pPLcZCY1, but it contains the complete lacY gene as well as the lacA gene. This plasmid contains the coding sequence for a fusion protein between the first 99 amino acids of MS2 polymerase and β-galactosidase. Expression of a carboxy terminal lacZ and an amino terminal MS2 polymerase (99 amino acids) fused protein is possible via the BamHI site (phase for both fusions: GAT). The natural EcoRI site at the end of lacZ gene was removed by mutation. Other name of the plasmid is pPLc236LacPol2. |
EMBL Accession number: | - |
Latest sequence update: | 31/01/1989 |
Sequence detail: | Nucleotide sequence at the junction of the MS2 polymerase gene and the lacZ gene: 98 9 10 5' TGG.GAT.CCC.GTC.GTT 3' * BamHI ^ *: 98th codon of the MS2 polymerase gene. ^: 9th codon of the lacZ gene. Punctuation indicates the reading frame. |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr E. Remaut(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DS410λcIts (BA) |
Host reference: | - |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 28°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPLcZCYA1 (LMBP 488) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.