Last data update: 24 January 2024 16:39 CET
Plasmid name: pPLi342 (LMBP 1221)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
ppli342.gb
(View with Genome Compiler) ppli342.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Phage λ cI temperature-sensitive repressor gene (cIts, cI857) |
| Promoter: | Phage λ major leftward promoter (λ PL) Phage λ major rightward promoter (λ PR) Phage λ promoter for repressor maintenance (λ PRM) |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pAT153cI857; pPLi34 |
| Further information: | This plasmid contains the cI857 temperature-sensitive repressor gene. Designed for λ PL promoter-regulated expression of fragments containing a ribosome binding site functional in Escherichia coli. The orientation of the plasmid origin is clockwise (inverted relative to pBR, pPLc, pPLa plasmids). The plasmid displays temperature-dependent runaway replication. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727572.1. |
| EMBL Accession number: | LT727572.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 25/03/2011 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: EcoRI, NdeI/NsiI and XbaI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr E. Remaut(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPLi342 (LMBP 1221) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.