Last data update: 24 January 2024 16:39 CET
Plasmid name: pPNTNeoflox-msGCA1 (LMBP 4472)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p4472.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse guanylate cyclase 1, soluble, alpha 3 cDNA (Gucy1a3, alfa 1 sGC, sGC-alfa1, sGCA1); homology arms |
| Promoter: | Mouse phosphoglycerate kinase 1 promoter (PGK1) Escherichia coli lac operon promoter Phage T3 promoter |
| Ribosome binding site: |
- |
| Terminator: | Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA) Mouse phosphoglycerate kinase 1 polyadenylation signal (PGK1 polyA) |
| Selection marker: | Ampicillin (amp) Neomycin (neo; G418) Thymidine kinase (TK) |
| Replicon: | Escherichia coli plasmid pMB1/ColE1 origin |
| Host range: | Escherichia coli; preferably recombination-deficient strains Mammalian cells |
| Parental clone: | pPNT |
| Further information: | This plasmid was developed for 'knock-out' experiments in mammalian cells by double homologous recombination (positive and negative selection).When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. |
| EMBL Accession number: | - |
| Latest sequence update: | 16/05/2002 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BglI, NotI/XhoI and SmaI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr P. Brouckaert(1) (2). It was constructed by M. Buys(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - VIB/BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPNTNeoflox-msGCA1 (LMBP 4472) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr P. Brouckaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.