Last data update: 24 January 2024 16:39 CET
Plasmid name: pPP2E6F1 (LMBP 3199)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | not available |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Saccharomyces cerevisiae α-mating factor 1 gene (MFα1); prepro secretion signal sequence (ppMF) Mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ) cDNA; Fab fragment (E6F1) |
Promoter: | Pichia pastoris alcohol oxidase 1 promoter (AOX1) |
Ribosome binding site: |
- |
Terminator: | Pichia pastoris alcohol oxidase 1 terminator (AOX1) |
Selection marker: | Ampicillin (amp) Pichia pastoris HIS4; auxotrophic |
Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
Host range: | Escherichia coli Pichia pastoris; his4(-), integrative |
Parental clone: | pPIC9 |
Further information: | The plasmid is used for the expression of the Fab fragment (E6F1) of the mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ). |
EMBL Accession number: | - |
Latest sequence update: | 02/06/2003 |
Authenticity test: | By lack of sequence data, it was not possible to perform the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Dr K. De Sutter(1) (2) and Prof. Dr R. Contreras(1) (2). It was constructed by W. Lammerant(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium. |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 MC1061 |
Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPP2E6F1 (LMBP 3199) is available at BCCM/GeneCorner. This plasmid was deposited by Dr K. De Sutter and Prof. Dr R. Contreras . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.