Last data update: 24 January 2024 16:39 CET
Plasmid name: pPSA2 (LMBP 2885)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p2885.gb
(View with Genome Compiler) p2885.txt p2885.pdf |
| Sequence analysis results Genecorner: |
Sanger: 148921.fasta Sanger: 148922.fasta NGS: a05-gc-dec2018.fasta |
| Cloned DNA: |
Staphylococcus aureus protein A cDNA (spa); fragment |
| Promoter: | Staphylococcus aureus protein A promoter (psa) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Staphylococcus aureus protein A (spa) gene |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) Chloramphenicol (cam) |
| Replicon: | Escherichia coli plasmid pMB1 origin Staphylococcal plasmid pC194 origin |
| Host range: | Escherichia coli Staphylococcus aureus |
| Parental clone: | pRIT21 |
| Further information: | The plasmid was constructed based on pRIT21 by introducing an SpeI site upstream of the presumed Shine-Dalgarno sequence of the Staphylococcus aureus protein A (spa) gene and creating an AflIII site to introduce an ATG, replacing the wild type TTG of the spa gene. Genes of interest can be cloned into pPSA2 by opening the plasmid via AflIII/EcoRI restriction digest. The region upstream of the ampicillin resistance gene and downstream of the pMB1 ori have been sequenced at BCCM/GeneCorner. |
| EMBL Accession number: | - |
| Latest sequence update: | 22/06/2018 |
| Sequence detail: | Sequence at the start of the S. aureus protein A coding sequence:
5' GACTTTACTAGTACATACAGGGGGTATTAAC.ATG.TAA. 3'
* * ----------- * * *
SpeI S.D.
*: mutated nucleotide |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BglI/HindIII, EcoRI/PvuI, NcoI and SspI. This plasmid has also been fully sequenced but the NGS sequence data still need to be implemented. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr L. Steidler(1) (2) and Prof. Dr E. Remaut(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Steidler et al., Appl. Environ. Microbiol. 62 (1996), 2356-2359 [PMID: 8779573] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) + chloramphenicol (34 μg/ml)* |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | *: selection of transformants on chloramphenicol; subsequent cultivation of a single colony in liquid medium with ampicillin. |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPSA2 (LMBP 2885) is available at BCCM/GeneCorner. This plasmid was deposited by Dr L. Steidler and Prof. Dr E. Remaut and was published in Steidler et al., 1996. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.