Last data update: 24 January 2024 16:39 CET
Plasmid name: pPpT4-NSS-PcGH61D (LMBP 12117)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
NGS: b05-gc-may2021.fasta |
| Cloned DNA: |
Phanerochaete chrysosporium lytic polysaccharide monooxygenase 9D cDNA (LPMO9D, GH61D) Histidine tag (His-tag); C-terminal |
| Promoter: | Pichia pastoris alcohol oxidase 1 promoter (AOX1) Synthetic prokaryotic EM72 promoter Pichia ketol-acid reductoisomerase promoter (ILV5) |
| Ribosome binding site: |
- |
| Terminator: | Pichia pastoris alcohol oxidase 1 terminator (AOX1) Pichia pastoris alcohol oxidase terminator (AOD) |
| Selection marker: | Bleomycin (bleo; zeomycin (zeo; Zeocin); phleomycin (phleo)) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Pichia pastoris; integrative |
| Parental clone: | pPpT4 |
| Further information: | The plasmid was constructed by PCR amplifying the P. chrysosporium GH61D coding sequence and cloning it into the E. coli - P. pastoris shuttle vector pPpT4. The P. chrysosporium GH61D coding sequence is preceded by its native secretion signal and was codon-optimised for P. pastoris. The plasmid is also available for expression in P. pastoris, integrated in the genomic DNA, under accession number LMBP 12113. The nucleotide sequence of the P. chrysosporium GH61D coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number BAL43430.1. The nucleotide sequence of the pPpT4 parental vector corresponds with the EMBL Nucleotide Sequence Database accession number JQ519689.1 except for some nucleotides upstream of the AOX1 promoter. Other name of the plasmid is pPpT4-NSS-PcGH61D (sExtra112). |
| EMBL Accession number: | BAL43430.1, view at EMBL, GenBank, DDBJ JQ519689.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 05/06/2020 |
| Authenticity test: | This plasmid has been fully sequenced but the NGS sequence data still need to be implemented. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Z. Ubiparip(1) and Prof. Dr T. Desmet(1). It was constructed by Dr B. Danneels(1). (1) Center for Synthetic Biology, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium |
| Plasmid reference: | Danneels et al., Biotechnol. J. 14 (2019), e1800211 [PMID: 30238672] [DOI: 10.1002/biot.201800211] |
| Related plasmid reference: | Tanghe et al., Mol. Biotechnol. 57 (2015), 1010-1017 [PMID: 26285758] [DOI: 10.1007/s12033-015-9887-9] |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αT1R |
| Host reference: | - |
| Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
| Cultivation medium: | LB-Lennox + zeocin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | LMBP 12113 |
Refer in your Materials and Methods: |
pPpT4-NSS-PcGH61D (LMBP 12117) is available at BCCM/GeneCorner. The plasmid was deposited by Z. Ubiparip and Prof. Dr T. Desmet and was published in Danneels et al., 2019. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.