Last data update: 24 January 2024 16:39 CET
Plasmid name: pPpT4alpha-VSV-ADIP-hMALT1Cd100-His (LMBP 9395)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p9395.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human MALT1 paracaspase cDNA (MALT1, MLT, PCASP1, paracaspase 1); fragment Mouse adiponectin, C1Q and collagen domain containing cDNA (Adipoq, Acdc, Acrp30, Ad, adipo, adiponectin, apM1, APN, GBP28); fragment Saccharomyces cerevisiae α-mating factor 1 gene (MFα1); prepro secretion signal sequence (ppMF) Vesicular stomatitis virus glycoprotein (VSV-G) epitope; N-terminal Histidine tag (His-tag); C-terminal |
| Promoter: | Pichia pastoris alcohol oxidase 1 promoter (AOX1) Synthetic prokaryotic EM72 promoter Pichia pastoris ILV5 promoter |
| Ribosome binding site: |
- |
| Terminator: | Pichia pastoris alcohol oxidase 1 terminator (AOX1) Pichia pastoris anti-oxidative defense gene terminator (AOD) |
| Selection marker: | Bleomycin (bleo; zeomycin (zeo; Zeocin); phleomycin (phleo)) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Pichia pastoris |
| Parental clone: | pPpT4alpha-VSV-hMALT1Cd100-His |
| Further information: | The plasmid was constructed by cloning the VSV-ADIP sequence into Xho/EcoRV opened pPpT4alpha-VSV-hMALT1Cd100-His vector, upstream of the human MALT1 fragment. Adiponectin is a multimerization domain (Mashalidis et al., 2013) meant to increase in vitro activity in low salt conditions. The plasmid expresses the minimal paracaspase domain of human MALT1, in frame of the S. cerevisiae ppMF, wich means that Pichia induced by methanol will secrete the protein construct. The expressed protein can be purification via the C-terminal His-tag. The N-terminal VSV tag allows verification that the purified product is intact. The nucleotide sequence of the human MALT1 coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number AF130356.2. Other name of the plasmid is pPpT4alpha-VSV-ADIP-MALT1Cd100-His. |
| EMBL Accession number: | AF130356.2, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 12/04/2017 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by DR J. Staal(1)(2) and Prof. Dr R. Beyaert(1)(2). (1) VIB Center for Inflammation Research, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Mashalidis et al., Anal. Biochem. 437 (2013), 150-160 [PMID: 23439381] [DOI: 10.1016/j.ab.2013.02.008] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + zeocin (25 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPpT4alpha-VSV-ADIP-hMALT1Cd100-His (LMBP 9395) is available at BCCM/GeneCorner. The plasmid was deposited by DR J. Staal and Prof. Dr R. Beyaert. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.