Last data update: 24 January 2024 16:39 CET
Plasmid name: pPpT4alphaS-F-MALT1C-ΔC100-MycHis (LMBP 8946)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human MALT1 paracaspase cDNA (MALT1, MLT, PCASP1, paracaspase 1, GeneID 10892); fragment FLAG epitope tag; N-terminal Myc epitope; C-terminal Histidine tag (His-tag); C-terminal Saccharomyces cerevisiae α-mating factor 1 gene (MFα1); prepro secretion signal sequence (ppMF) |
| Promoter: | Pichia pastoris alcohol oxidase 1 promoter (AOX1) Synthetic prokaryotic EM72 promoter Pichia pastoris ILV5 promoter |
| Ribosome binding site: |
- |
| Terminator: | Pichia pastoris alcohol oxidase 1 terminator (AOX1) Pichia pastoris anti-oxidative defense gene terminator (AOD) |
| Selection marker: | Bleomycin (bleo; zeomycin (zeo; Zeocin); phleomycin (phleo)) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Pichia pastoris |
| Parental clone: | pPpT4-Alpha-S; pCD-F-MALT1C-ΔC100-MycHis |
| Further information: | The plasmid was constructed by PCR amplifying the human MALT1 fragment from pCD-F-MALT1C-ΔC100-MycHis, including the N-terminal FLAG-tag and C-terminal myc- and His-tags, and cloning it into the SnaBI/NotI opened pPpT4-Alpha-S vector. This plasmid contains a C-terminal part of the human MALT1 coding sequence, from which the final 100 amino acids have been deleted. Other name of the plasmid is pPpT4alphaS-F-MALT1C-d100-myc/his. |
| EMBL Accession number: | - |
| Latest sequence update: | 18/07/2019 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr J. Staal(1) (2) and Prof. Dr R. Beyaert(1) (2). (1) UGent-VIB Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + zeocin (25 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPpT4alphaS-F-MALT1C-ΔC100-MycHis (LMBP 8946) is available at BCCM/GeneCorner. This plasmid was deposited by Dr J. Staal and Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.