Last data update: 24 January 2024 16:39 CET
Plasmid name: pQE2-hHIPK2-781-1191 (LMBP 9792)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p9792.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human homeodomain interacting protein kinase 2 cDNA (HIPK2); fragment Escherichia coli lac repressor gene (lacI) Histidine tag (His-tag); N-terminal |
| Promoter: | Phage T5 N25 promoter with two lac operator sequences (N25/O2) Escherichia coli lac repressor promoter; mutant (lacI(q)) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | Escherichia coli rrnB operon T1 terminator |
| Selection marker: | Ampicillin (amp) Chloramphenicol (cam) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pQE-2 |
| Further information: | The plasmid was constructed by isolating the C-terminal fragment of the human HIPK2 coding sequence via PmlI/HindIII restriction, and cloning it into the PmlI/HindIII opened pQE-2 vector. The C-terminal fragment of the human HIPK2 coding sequence consists of amino acids 789 up to 1198, following EMBL accession number AF208291. The nucleotide sequence of the human HIPK2 coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number AF208291.1. Other name of the plasmid is pQE2-HIPK2 781-1191. |
| EMBL Accession number: | AF208291.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 22/01/2016 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr L. Schmitz(1). (1) Institute of Biochemistry, Faculty of Medicine, Justus-Liebig-University, Giessen, Germany |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pQE2-hHIPK2-781-1191 (LMBP 9792) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr L. Schmitz. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.