Last data update: 24 January 2024 16:39 CET
Plasmid name: pQE30-N-tag-ProP439-500-I474P (LMBP 12392)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Escherichia coli osmolyte:H+ symporter ProP cDNA (proP, GeneID 948626); mutated fragment MRGSH6-FactorX cDNA; N-terminal |
| Promoter: | Phage T5 N25 promoter with two lac operator sequences (N25/O2) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator Phage T4 gene 32 terminator (T4g32) |
| Selection marker: | Ampicillin (amp) Chloramphenicol (cam) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pQE30-N-tag-ProP439-500 |
| Further information: | The plasmid was constructed by introducing an I474P mutation into the E. coli ProP coding sequence of the pQE30-N-tag-ProP439-500 vector. The plasmid encodes amino acid position 439-500 of the E. coli ProP, fused at its N-terminus to the vector-encoded peptide MRGSH6-FactorX (N-tag), which has amino acid sequence MRGSHHHHHHGSGSGSGIEGR. The nucleotide sequence of the wild type E. coli ProP cDNA corresponds with the EMBL Nucleotide Sequence Database accession number M83089.1. Other name of the plasmid is pDC315. |
| EMBL Accession number: | M83089.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 10/12/2020 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr D.E. Culham(1) and Prof. Em. Dr J.M. Wood(1). (1) Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada |
| Plasmid reference: | Romantsov et al., Mol. Microbiol. 103 (2017), 469-482 [PMID: 27802568] [DOI: 10.1111/mmi.13569] |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 WG350 (LMBP 12428) |
| Host reference: | Culham et al., J. Mol. Biol. 229 (1993), 268-276 [PMID: 8421314] [DOI: 10.1006/jmbi.1993.1030] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pQE30-N-tag-ProP439-500-I474P (LMBP 12392) is available at BCCM/GeneCorner. This plasmid was deposited by Dr D.E. Culham and Prof. Em. Dr J.M. Wood and was published in Romantsov et al., 2017. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.