Last data update: 24 January 2024 16:39 CET
Plasmid name: pQE9-SMx1 (LMBP 9653)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p9653.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse MX dynamin-like GTPase 1 cDNA (Mx1, Mx-1, GeneID 17857) Histidine tag (His-tag); N-terminal |
| Promoter: | Phage T5 N25 promoter with two lac operator sequences (N25/O2) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | Escherichia coli rrnB operon T1 terminator |
| Selection marker: | Ampicillin (amp) Chloramphenicol (cam) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pQE9 |
| Further information: | The plasmid was constructed by cloning the Mus spretus Mx1 coding sequence into the BamHI/SalI opened pQE9 vector. This vector is meant for the inducible expression of Mus spretus Mx1 in E. coli. |
| EMBL Accession number: | - |
| Latest sequence update: | 11/02/2016 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr X. Saelens(1)(2)(3). (1) Department of Medical Protein Research, VIB, Ghent, Belgium (2) Inflammation Research Center, VIB, Ghent, Belgium (3) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Verhelst et al., J. Virol. 89 (2015), 10879-10890 [PMID: 26292322] [DOI: 10.1128/JVI.01744-15] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pQE9-SMx1 (LMBP 9653) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr X. Saelens and was published in Verhelst et al., 2015. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.