Last data update: 24 January 2024 16:39 CET
Plasmid name: pQEB1sFv (LMBP 2951)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p2951.gb
(View with Genome Compiler) p2951.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Erwinia carotovora pelB gene; signal sequence Mouse anti-BCL1 monoclonal antibody IgG1 cDNA; single chain Fv sequence (B1sFv) Histidine tag (His-tag); C-terminal |
| Promoter: | Phage T5 N25 promoter with two lac operator sequences (N25/O2) |
| Ribosome binding site: |
Ribosome binding site RBSII; synthetic sequence |
| Terminator: | Phage λ T0 terminator Escherichia coli rrnB operon T1 terminator |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli; use strains supplying the LacI repressor |
| Parental clone: | pQE-6; pQE-12 |
| Further information: | This plasmid allows the high-level production of a recombinant single chain Fv fragment of the mouse IgG1 antibody against the BCL1 antigen. The recombinant product contains a 6xHis-tag at the carboxy terminus. The promoter/operator element T5 N25/O2 consists of the highly efficient E. coli phage T5 N25 promoter and two lac operator sequences (Bujard et al. 1987). The presence of the lacI(q) repressor gene is necessary to control the activity of the promoter. The synthetic ribosome binding site RBSII is described in Stüber et al. (1990). The actual sequences of the ribosome binding site RBSII in front of the pelB signal sequence as well as the junction between pelB and scFv have been experimentally verified. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727560.1. |
| EMBL Accession number: | LT727560.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 29/11/1993 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: EcoRI and Tth111I. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr J. De Jonge(1). (1) Department of Immunology-Physiology, VUB, Brussels, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Stüber et al., Eur. J. Immunol. 20 (1990), 819-824 [PMID: 1693336] Bujard et al., Methods Enzymol. 155 (1987), 416-433 [PMID: 2828874] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 RR1ZΔM15 |
| Host reference: | Altieri et al., J. Bacteriol. 168 (1986), 648-654 [PMID: 2946661] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pQEB1sFv (LMBP 2951) is available at BCCM/GeneCorner. This plasmid was deposited by Dr J. De Jonge. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.